Penicilina-estreptomicina (10.000 U/ml)

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Penicilina-estreptomicina (10.000 U/ml)

Esta solución contiene 10.000 unidades/ml de penicilina y 10.000 µg/ml de estreptomicina. Los antibióticos penicilina y estreptomicina se usan paraMás información

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Número de catálogo	Cantidad
15140122 también denominado 15140-122	100 mL
15140148 también denominado 15140-148	20 mL
15140163 también denominado 15140-163	20 x 100 mL

3 opciones

Número de catálogo 15140122

también denominado 15140-122

Precio (EUR)

20,92

Each

En Stock

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Cantidad:

100 mL

Precio (EUR)

20,92

Each

Añadir al carro de la compra

Esta solución contiene 10.000 unidades/ml de penicilina y 10.000 µg/ml de estreptomicina. Los antibióticos penicilina y estreptomicina se usan para impedir la contaminación bacteriana de los cultivos celulares debido a su eficaz acción combinada contra bacterias grampositivas y gramnegativas. La penicilina se purificó en un principio a partir de los hongos Penicillium y actúa mediante una interferencia directa con el volumen de la pared celular bacteriana, e indirecta mediante la activación de la liberación de enzimas que alteran aún más la pared celular. La estreptomicina fue originalmente purificada a partir de Streptomyces griseus. Actúa mediante la creación de una unión a la subunidad 30S del ribosoma bacteriano, lo que inhibe la síntesis de proteínas y la muerte de las bacterias susceptibles.

Ofrecemos una amplia gama de antibióticos y antimicóticos tanto en formato líquido como en polvo. Obtenga más información sobre los siguientes tipos de productos:

• Antibióticos de cultivo celular
• Antibióticos de selección (incluidas las concentraciones de trabajo recomendadas)

Obtenga más información sobre el uso de antibióticos y antimicóticos en cultivo celular y revise las directrices para la descontaminación de cultivos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Concentración100X

Para utilizar con (aplicación)Prevención de la contaminación del cultivo celular

Cantidad100 mL

Duración de almacenamiento12 meses

Condiciones de envíoHielo seco

FormularioLíquido

Tipo de productoPenicilina-estreptomicina

EsterilidadEstéril con filtro

Sterilization MethodSterile-filtered

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De – 5 °C a – 20 °C
Condiciones de envío: Hielo seco
Vida útil: 12 meses a partir de la fecha de fabricación

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Preguntas frecuentes

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.