El suplemento B-27 para el cultivo celular de neuronas es el más citado; es un suplemento optimizado sin suero queMás información
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Número de catálogo
Cantidad
17504001
100 mL
17504044
10 mL
2 opciones
Número de catálogo 17504001
Precio (EUR)
1.120,00
Each
Añadir al carro de la compra
Cantidad:
100 mL
Precio (EUR)
1.120,00
Each
Añadir al carro de la compra
El suplemento B-27 para el cultivo celular de neuronas es el más citado; es un suplemento optimizado sin suero que se utiliza para favorecer el crecimiento de baja o alta densidad y la viabilidad a corto o largo plazo de neuronas del hipocampo embrionarias, posnatales y adultas, así como otras neuronas del SNC. El suplemento B-27 sin vitamina A se suministra como un líquido 50X y se ha diseñado para su uso con medios Neurobasal o Neurobasal-A para realizar cultivos celulares de neuronas sin necesidad de una capa subyacente de astrocitos.
El suplemento B-27 es adecuado para su uso en la mayoría de las aplicaciones de células neuronales. Para otras aplicaciones, ofrecemos formulaciones alternativas del Suplemento B-27 y medios Neurobasal.
Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.
Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
What supplements should I use with Neurobasal or Neurobasal-A medium?
Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .
We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.
For more information on Neurobasal media, search "Neurobasal" from our website home page.
Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?
We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.