1x1 image pixel for data collection
Suplemento B-27™ (50x), sin suero
Suplemento B-27™ (50x), sin suero
Gibco™

Suplemento B-27™ (50x), sin suero

El suplemento B-27 para el cultivo celular de neuronas es el más citado; es un suplemento optimizado sin suero queMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidad
17504001100 mL
1750404410 mL
Número de catálogo 17504001
Precio (EUR)
1.120,00
Each
Añadir al carro de la compra
Cantidad:
100 mL
Precio (EUR)
1.120,00
Each
Añadir al carro de la compra
El suplemento B-27 para el cultivo celular de neuronas es el más citado; es un suplemento optimizado sin suero que se utiliza para favorecer el crecimiento de baja o alta densidad y la viabilidad a corto o largo plazo de neuronas del hipocampo embrionarias, posnatales y adultas, así como otras neuronas del SNC. El suplemento B-27 sin vitamina A se suministra como un líquido 50X y se ha diseñado para su uso con medios Neurobasal o Neurobasal-A para realizar cultivos celulares de neuronas sin necesidad de una capa subyacente de astrocitos.

El suplemento B-27 es adecuado para su uso en la mayoría de las aplicaciones de células neuronales. Para otras aplicaciones, ofrecemos formulaciones alternativas del Suplemento B-27 y medios Neurobasal.

Para protocolos establecidos que usen el suplemento B-27, acceda a nuestro manual de protocolos de neurobiología Gibco.

Vea las publicaciones que utilizan productos de neurobiología Gibco en nuestra CellCite Gibco.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Tipo de célulaCélulas neuronales
Concentración10 X
Tipo de cultivoCultivo de células de mamífero
Cantidad100 mL
Nivel de sueroSin suero
Duración de almacenamiento12 meses
Probado paraEndotoxinas, rendimiento, esterilidad, micoplasma
ClasificaciónSin suero
FormularioLíquido
Tipo de productoSuplemento de cultivo celular
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 20°C) y proteger de la luz.

Preguntas frecuentes

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Documentos y descargas

Certificados

Hojas de datos de seguridad

Compartir número de catálogo, nombre o enlace.