ReadyProbes™ Reagent F-Actin Phalloidin Conjugates
ReadyProbes™ Reagent F-Actin Phalloidin Conjugates
Invitrogen™

ReadyProbes™ Reagent F-Actin Phalloidin Conjugates

Perfect for fixed cells and tissue, ReadyProbes Reagent F-actin phalloidin conjugates offer clear visualization of actin filaments, making it perfect for cell biology, neuroscience, cancer research, and more.
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Número de catálogoColorIntervalo de longitud de onda de excitación
R37110Verde495/518
R37112Rojo-naranja540/565
Número de catálogo R37110
Precio (EUR)
333,00
Each
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Color:
Verde
Intervalo de longitud de onda de excitación:
495/518
Precio (EUR)
333,00
Each
Añadir al carro de la compra
Ask our AI about this Product
ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle.

These ready-to-use solutions combines superior brightness with exceptional F-actin staining. Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

Features

  • High-affinity staining of F-actin with superior specificity compared to antibody methods
  • ActinGreen 488 Excitation/Emission: (495/518 nm), detected through standard GFP and FITC filter sets
  • ActinRed 555 Excitation/Emission: (540/565 nm), detected through standard RFP and TRITC filters sets
  • Use counterstain (i.e., DAPI or NucBlue ReadyProbes reagent) as desired

ReadyProbes format advantages

  • Ready-to-use formulation—no need to dilute, weigh, or pipette
  • Just use two drops per mL of medium to stain, incubate 30 minutes, wash, then image
  • Stable at room temperature—keep handy on the benchtop

Applications

  • For fixed and permeabilized cells or tissue
  • Fluorescence microscopy (IF/IHC/ICC)
  • High content screening, high content imaging
  • Flow cytometry

Suggestions for use

  • Both ActinRed 555 and ActinGreen 488 reagents may be added directly to fixed cells in full medium or buffer solutions
  • In most cases 2 drops/mL and an incubation time of 15–30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
ColorVerde
DescripciónActinGreen™ 488 ReadyProbes™ Reagent (AlexaFluor™ 488 phalloidin)
Método de detecciónFluorescente
Tipo de coloranteAlexa Fluor™ 488
EmisiónVisible
Intervalo de longitud de onda de excitación495/518
Para utilizar con (equipo)Microscopio confocal, sistema de adquisición de imagen de células Floid™, microscopio de fluorescencia, citómetro de flujo, sistema de imagen de células Floid™
FormularioLíquido
Línea de productosActinGreen™, ReadyProbes™
Cantidad2 frascos cuentagotas
TécnicaIntensidad de fluorescencia
Tipo de etiquetaFluorescent Dye
Tipo de productoFaloidina
SubCellular LocalizationCitoesqueleto
Unit SizeEach
Contenido y almacenamiento
2 frascos cuentagotas de 2,5 ml

Almacenar a ≤ 25 °C.

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Preguntas frecuentes

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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