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Gibco™
TrypLE™ Select Enzyme (1X), no phenol red
Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.
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| Catalog Number | Quantity |
|---|---|
| 12563029 | 500 mL |
| 12563011 | 100 mL |
| 12563030 | 5 L |
| 12563031 | 10 L |
Catalog number 12563029
Price (EUR)
161,00
Each
-
Quantity:
500 mL
Price (EUR)
161,00
Each
Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, TrypLE Select is:
- Gentle on cells—maintains cell health for reproducible results
- Room-temperature stable—convenient to use
- Easy to use—substitutes directly into existing protocols with no inactivation required
- Animal origin free (AOF)—pure, consistent, and effective
This TrypLE Select is modified as follows:
With
- EDTA
Without
- Phenol Red
Gentle on cells
TrypLE Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE Select is room-temperature stable and ready to use. TrypLE Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE Select is free of animal-derived components and is formulated on dedicated animal origin-free equipment.
Product Intended Use
Customers using TrypLE Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
TrypLE Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1 X
Culture TypeAdherent Cell Culture
Endotoxin LevelLow
FormatBottle(s)
Green FeaturesEnergy efficient, Sustainable packaging
Osmolality280 - 300 mOsm/kg
Product LineGibco™
Quantity500 mL
RemarksRoom Temperature Stable
Shelf Life24 Months
Shipping ConditionRoom Temperature
SourceAnimal origin-free
Tested ForIn vitro Bioassay
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
Without AdditivesNo Phenol Red
pH7 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C, protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3172538Certificate of AnalysisJun 27, 202512563029, 12563011
3118208Certificate of AnalysisJun 07, 202512563029, 12563011
3118206Certificate of AnalysisMay 23, 202512563029, 12563011
3007524Certificate of AnalysisMar 29, 202512563029, 12563011
2898849Certificate of AnalysisMar 28, 202512563029, 12563011
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Limited Use Label Licenses (LULL)
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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product for mammailian and insect cell culture only, to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component (including, but not limited to, direct use for therapeutic purposes, use as an excipient in finished drug formulations, or use as an active pharmaceutical ingredient); (3) use the product in any applications for direct topical or in vivo use; (4) use the product in any applications for in vitro fertilization; (5) reverse engineer the product or cause the product to be reverse engineered; or (6) use the product for purposes other than what is indicated in this Limited Use Label License. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
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