DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA.Application: Labeling DNA withRead more
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Catalog number 18162016
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DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA. Application: Labeling DNA with either radiolabeled or biotinylated nucleotides. Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964. Performance and Quality Testing: Performance tested in nick translation reaction. Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer. Unit Reaction Conditions: 50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/ml template•primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDNA Polymerase
PolymeraseDNA Polymerase I
Exonuclease Activity3' - 5', 5' - 3'
Quantity250 U
Shipping ConditionWet Ice
Hot StartNo
Unit Size250 units
Contents & Storage
DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.