DMEM/F-12, powder
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Gibco™

DMEM/F-12, powder

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth ofRead more
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Catalog NumberQuantity
3250003510 L
32500043
also known as 32500-043
50 L
Catalog number 32500035
Price (EUR)
54,50
Each
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Quantity:
10 L
Customize this product
Price (EUR)
54,50
Each
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Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM / F-12 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate


The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications
ClassificationAnimal Origin-free
FormPowder
Product TypeDMEM/F-12
Serum LevelStandard Serum Supplementation
Shelf Life36 Months
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES, No Sodium Bicarbonate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 L
Shipping ConditionRoom Temperature
Osmolality250 - 290 mOsm/kg
pH6.2 to 6.6
Culture EnvironmentCO2
Culture TypeMammalian Cell Culture
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.

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