SYBR™ Green Universal Master Mix

Notification of updated SYBR Green master mix formulation: PowerTrack SYBR Green Master Mix is our newest formulation designed to greatly simplify your work and deliver high sensitivity and specificity. It comes with an optional yellow dye that can be added to your DNA sample for pipetting QC. Yellow sample added to blue master mix yields green qPCR reaction. Visually confirm that your DNA sample was added to master mix. PowerTrack SYBR Green Master Mix is compatible with a wide range of primer melting temperatures (55°C to 65°C) and concentrations to minimize primer optimization work.

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SYBR Green PCR Master Mix
Everything you need for SYBR Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• Premixed components stored at 2–8°C significantly reduce assay setup time
• SYBR Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability

Maximum flexibility and convenience
Applied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan probes are required. SYBR Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.