Contents & Storage
Kit content for Bac-to-Bac™ N-His TOPO™ cloning kit (For details see the manual)
• Vector kit for 20 topo cloning reactions:
o pFastBac⁄NT-TOPO™ Vector containing the N-terminal His-Tag and TEV cleavage site.
o pFastBac⁄NT-Gus Control Plasmid (Control expression vector)
o Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer
• Kit containing competent cells (20 reactions):
o One Shot™ Mach1-T1R Chemically Competent E. coli
Vector kit: store at -20°C
One Shot™ Mach1-T1R Chemically Competent E. coli: store at -80°C