AAV-MAX Transfection Kit
AAV-MAX Transfection Kit
Gibco™

AAV-MAX Transfection Kit

The AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.
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Catalog NumberQuantity
A505151 kit for 1 L of production
A505161 kit for 10 L of production
Catalog number A50515
Price (EUR)
924,65
Online Exclusive
1 000,00
Save 75,35 (8%)
Each
Add to cart
Quantity:
1 kit for 1 L of production
Price (EUR)
924,65
Online Exclusive
1 000,00
Save 75,35 (8%)
Each
Add to cart

The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, which are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium.

Features of the AAV-MAX Transfection Kit include:

  • Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • AAV-MAX transfection reagent—cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures
  • AAV-MAX booster—chemically defined component that works with the transfection reagent to achieve high transfection efficiency
  • AAV-MAX enhancer—chemically defined component that enhances the production of AAV particles
  • Robust transfection protocols are adaptable to large-scale transfection
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineHEK293
Culture TypeSuspension Cell Culture
DescriptionSerum-free, animal origin-free, chemically defined
Expression SystemMammalian
For Use With (Application)AAV Production
For Use With (Equipment)AAV-MAX System
Includes10 mL Gibco AAV-MAX Enhancer, 6 mL Gibco AAV-MAX Transfection Reagent, 3 mL Gibco AAV-MAX Supplement
Product LineAAV-MAX
Product TypeAAV production transfection Kit
Quantity1 kit for 1 L of production
Reagent TypeTransfection kit
Shelf Life12 months from date of manufacture
Shipping ConditionWet Ice
SterilitySterile
Cell TypeEstablished Cell Lines
FormatBottle
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
• 6 mL AAV-MAX Trasfection Reagent
• 3 mL AAV-MAX Transfection Booster
• 10 mL AAV-MAX Enhancer

Reagents should be stored at 2–8°C. Do not freeze.

Frequently asked questions (FAQs)

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Enhancer and what does it do?

The AAV-MAX Enhancer increases AAV vector production in Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the AAV-MAX Enhancer are proprietary.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Transfection Booster and what does it do?

The AAV-MAX Transfection Booster enhances the efficiency of co-transfection of multiple plasmids into high-density Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the transfection booster are proprietary.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Documents & Downloads

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