Ambion™ T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminatedRead more
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Catalog number AM2141
Price (EUR)/ 2,500 units
367,00
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Price (EUR)/ 2,500 units
367,00
Add to cart
Ambion™ T4 RNA Ligase, cloned, 5 U/μL
Catalog numberAM2141
Price (EUR)/ 2,500 units
367,00
-
Add to cart
Ambion™ T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 2,500 U (5 U/ μL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules.
Unit Definition: One unit catalyzes the formation of 1 nmol of [5'-32P]-rA12-18 into a phosphatase-resistant form in 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5 U/μL
FormatTube
EnzymeLigase
Fidelity (vs. Taq)10 X
No. of Reactions250
PolymeraseSP6 RNA Polymerase
Product LineAmbion™
Product TypeT4 Rna Ligase
Quantity5 μL
Shipping ConditionDry Ice
SourceRNA
FormSolution
Unit Size2,500 units
Contents & Storage
T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C.