Thermo Scientific Chemicals
Giemsa Stain
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10 g
50 g
Catalog number B21172.09
also known as B21172-09
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Quantity:
10 g
Request bulk or custom format
Price (EUR)/ Each
24,30
Online exclusive
27.00 
Save 2,70 (10%)
Add to cart
Giemsa Stain
Catalog numberB21172.09
Price (EUR)/ Each
24,30
Online exclusive
27.00 
Save 2,70 (10%)
-
Add to cart
Chemical Identifiers
CAS51811-82-6
IUPAC Name7-amino-N,N-dimethyl-3H-phenothiazin-3-iminium chloride
Molecular FormulaC14H14ClN3S
InChI KeyNALREUIWICQLPS-UHFFFAOYSA-N
SMILES[Cl-].C[N+](C)=C1C=CC2=NC3=CC=C(N)C=C3SC2=C1
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SpecificationsSpecification SheetSpecification Sheet
FormPowder
Specific Absorbance (E 1%/1cm)≥600 (Lambda-max @ 522-526nm)
Appearance (Color)Dark green or dark brown to black
lambda-max (UV-Vis)522-526nm (in methanol)
lambda-max (UV-Vis)640-652nm (in methanol)
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Giemsa Stain is used as a laboratory reagent and stain for microscopy. It is also used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain is also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Giemsa Stain is used as a laboratory reagent and stain for microscopy. It is also used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain is also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain.

Solubility
Insoluble in water (<0.1%), methanol (5.8 mg/L), and ethanol.

Notes
Keep container tightly sealed. Store in cool, dry conditions in well sealed containers. Incompatible with oxidizing agents.
RUO – Research Use Only

General References:

  1. K. K. Friend.; S. Chen.; F. H. Ruddle. Differential staining of interspecific chromosomes in somatic cell hybrids by alkaline Giemsa stain. Somatic Cell Genetics. 1976, 2 (2), 183-188.
  2. Julie R. Korenberg.; Elizabeth F. Freedlender. Giemsa technique for the detection of sister chromatid exchanges. Ind. Eng. Chem. Res. 1974, 48 (4), 355-360.