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            • Primary Antibodies ›
            • MED30 Antibodies

            Proteintech

            MED30 Monoclonal Antibody (1D7C4), CoraLite® Plus 488

            View all (10) MED30 antibodies
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            Catalog # CL488-67038
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            Price (EUR)
            548,00
            100 µL
             
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            Cite MED30 Monoclonal Antibody (1D7C4), CoraLite® Plus 488

            • Antibody Testing Data (1)
            Application
            MED30 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            MED30 Antibody (CL488-67038) in Flow

            1X10^6 HeLa cells were intracellularly stained with 0.4 µg CoraLite® Plus 488 Anti-Human MED30 (CL488-67038, Clone:1D7C4) (red), or 0.4 µg Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
            View Product

            MED30 Antibody in Flow Cytometry (Flow)

            Product Details

            CL488-67038

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            0.4 µg/1x10^6 cell suspension
            -
            Product Specifications

            Species Reactivity

            Human, Pig, Rat

            Host/Isotype

            Mouse / IgG2a

            Class

            Monoclonal

            Type

            Antibody

            Clone

            1D7C4

            Immunogen

            Recombinant protein
            View immunogen

            Conjugate

            CoraLite® Plus 488
            • Unconjugated
            • Request custom conjugation

            Excitation/Emission Max

            493/522 nm

            Form

            Liquid

            Purification

            Protein A

            Storage buffer

            PBS with 0.5% BSA, 50% glycerol

            Contains

            0.05% ProClin 300

            Storage conditions

            -20°C, store in dark

            Shipping conditions

            Wet ice

            Product Specific Information

            Immunogen sequence: MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN ENCGGMDPIP VEQLIPYVEE DGSKNDDRAG PPRFASEERR EIAEVNKKLK QKNQQLKQIM DQLRNLIWDI NAMLAMRN

            Target Information

            Med30 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. The mediator complex functions as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, and is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Found in the nucleus, Med30 is expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: Mediator complex subunit 30; Mediator of RNA polymerase II transcription subunit 30; putative mediator of RNA polymerase II transcription subunit 30; thyroid hormone receptor associated protein 6; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; TRAP/Mediator complex component TRAP25; Trap25

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            Gene Aliases: MED30; MED30S; THRAP6; TRAP25

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            UniProt ID: (Human) Q96HR3

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            Entrez Gene ID: (Pig) 100519234, (Human) 90390, (Rat) 299905

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            Function(s)

            RNA polymerase II transcription cofactor activity transcription cofactor activity receptor activity protein binding ligand-dependent nuclear receptor transcription coactivator activity vitamin D receptor binding thyroid hormone receptor binding ubiquitin protein ligase activity general transcription factor

            Process(es)

            regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter protein ubiquitination stem cell population maintenance intracellular steroid hormone receptor signaling pathway androgen receptor signaling pathway positive regulation of transcription, DNA-templated
            It has to be done as per old AB suggested Products section.
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