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Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
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| Catalog Number | No. of Reactions |
|---|---|
| F553S | 50 Reactions |
| F553L | 200 Reactions |
Catalog number F553S
Price (EUR)/ 50 reactions
85,75
-
No. of Reactions:
50 Reactions
Price (EUR)/ 50 reactions
85,75
Phusion High-Fidelity PCR Kit
Catalog numberF553S
Price (EUR)/ 50 reactions
85,75
-
Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.
The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume.
Highlights
- High fidelity (52X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
- Increased product yields with minimal enzyme amounts
Applications
- High-fidelity PCR
- High throughput
- Amplification of difficult (GC-rich) templates
- Template generation for sequencing
- Multiplex PCR
- Long-range PCR
- Cloning & mutagenesis
- Microarray
Notes
- Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePhusion High-Fidelity DNA Polymerase
Reaction SpeedFast
Product TypeHigh-Fidelity PCR Kit
Shipping ConditionDry Ice
For Use With (Application)Standard PCR, High-fidelity PCR
Fidelity (vs. Taq)52X
Hot StartNo
No. of Reactions50 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)20 kb or less
Unit Size50 reactions
Contents & Storage
• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion GC & HF Buffer
• dNTP mix (10 mM each)
• Control template (λ DNA) and DNA size standard
• 1.3 kb & 10 kb primers (4 μM each)
• 50 mM MgCl2 solution and DMSO
Store at –20°C.
• 5X Phusion GC & HF Buffer
• dNTP mix (10 mM each)
• Control template (λ DNA) and DNA size standard
• 1.3 kb & 10 kb primers (4 μM each)
• 50 mM MgCl2 solution and DMSO
Store at –20°C.