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G418 disulfate, Cell Culture Reagent, Thermo Scientific ChemicalsG418 sulfate, geneticin, CAS # 108321-42-2, is an antibiotic closely related to Gentamicin B, the common aminoglycoside antibiotic.
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Catalog number J62671.03
also known as J62671-03
Price (EUR)/ Each
99,90
Online exclusive
111.00 Save 11,10 (10%)
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Quantity:
1 g
Price (EUR)/ Each
99,90
Online exclusive
111.00 Save 11,10 (10%)
G418 disulfate, Cell Culture Reagent, Thermo Scientific Chemicals
Catalog numberJ62671.03
Price (EUR)/ Each
99,90
Online exclusive
111.00 Save 11,10 (10%)
-
Chemical Identifiers
CAS108321-42-2
IUPAC Name(2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2R,3R,4R,5R)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
Molecular FormulaC20H44N4O18S2
InChI KeyUHEPSJJJMTWUCP-KETIWVBHNA-N
SMILESOS(O)(=O)=O.OS(O)(=O)=O.CN[C@@H]1[C@@H](O)[C@@H](O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H](C(C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)OC[C@]1(C)O
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Specifications
Specification Sheet
Specification SheetAssay (special)Biological assay (ED50 resistant): ? 2500 µg/ ml
Assay (special)Biological assay (ED50 sensitive): ? 400 µg/ ml
Potency? 720 units/ mg
Solution TestSolubility in 100 mg/ml: Clear to slightly hazy solution
Appearance (Color)White
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G418 disulfate is an aminoglycoside that is toxic to bacteria, yeast, protozoans and helminths. It prevents protein synthesis by blocking the elongation step in prokaryotic and eukaryotic ribosomes. It is used as a selection agent for G418 resistant bacteria or fungi after transformation.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General description
• G418 sulfate (geneticin) is an aminoglycoside antibiotic isolated from Micromonospora rhodorangea
• G418 is a bacteriostatic that prevents polypeptide formation by inhibiting the elongation step of polypeptide synthesis
Applications
• G418 sulfate can be used for selection of genetically engineered cells in both eukaryotes and prokaryotes
• Laboratory grade for applications such as biochemistry
RUO – Research Use Only
General References:
- Arshad, H.; Patel, Z.; Mehrabian, M.; Bourkas, M.; Al-Azzawi, Z.; Schmitt-Ulms, G., & Watts, J. C. 2021. The aminoglycoside G418 hinders de novo prion infection in cultured cells. The Journal of biological chemistry, 297(3), 101073.
- Ferguson, M. W.; Gerak, C.; Chow, C.; Rastelli, E. J.; Elmore, K. E.; Stahl, F.; Hosseini-Farahabadi, S.; Baradaran-Heravi, A.; Coltart, D. M., & Roberge, M. 2019. The antimalarial drug mefloquine enhances TP53 premature termination codon readthrough by aminoglycoside G418. PloS one, 14(5), e0216423.