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ViraPower™ II Lentiviral Gateway™ Expression System
The ViraPower™ II Lentiviral Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell Read more
Catalog number K36720
Price (EUR)/ 1 kit
3 247,00
Online exclusive
3820.00 Save 573,00 (15%)
-
Price (EUR)/ 1 kit
3 247,00
Online exclusive
3820.00 Save 573,00 (15%)
ViraPower™ II Lentiviral Gateway™ Expression System
Catalog numberK36720
Price (EUR)/ 1 kit
3 247,00
Online exclusive
3820.00 Save 573,00 (15%)
-
The ViraPower™ II Lentiviral Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ II Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.2⁄V5-DEST™ vector in the kit has the CMV promoter for driving constitutive expression of the target gene in mammalian cells. The ViraPower™ II Lentiviral system uses the PGK promoter instead of SV40 promoter in the lentiviral vector for driving long-term, persistent expression of the blasticidin stable selection marker.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable expression, long term expression
Key Features
• CMV promoter for high level, constitutive expression of the target gene
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit (Cat # V36820)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable expression, long term expression
Key Features
• CMV promoter for high level, constitutive expression of the target gene
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit (Cat # V36820)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeLentiviral
PromoterCMV
Product TypeLentiviral Expression System
Selection Agent (Eukaryotic)Blasticidin
Protein TagV5 Epitope Tag
Cloning MethodGateway
Quantity1 kit
VectorpLenti
Product LineGateway™, ViraPower™
For Use With (Application)Viral Expression
Unit Size1 kit
Contents & Storage
pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit
• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)
• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)
• One Shot™ Stbl3™ Chemically Competent E. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).
ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)
• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)
• 50 mg of Blasticidin powder (Cat # R21001)
• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.
Storage
• Vectors: -20°C
• ViraPower™ Packaging Mix: -20°C
• Blasticidin: -20°C
• Lipofectamine™ 2000: 4°C (do not freeze)
• One Shot™ Stbl3™ Chemically Competent E. coli:-80°C
• 293 FT Cells: Liquid Nitrogen
• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)
• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)
• One Shot™ Stbl3™ Chemically Competent E. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).
ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)
• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)
• 50 mg of Blasticidin powder (Cat # R21001)
• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.
Storage
• Vectors: -20°C
• ViraPower™ Packaging Mix: -20°C
• Blasticidin: -20°C
• Lipofectamine™ 2000: 4°C (do not freeze)
• One Shot™ Stbl3™ Chemically Competent E. coli:-80°C
• 293 FT Cells: Liquid Nitrogen