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Application
FIGURE: 1 / 1
SPAG11A Antibody (PA5-116883) in WB
Western blot analysis of SPAG11A in extracts of Rat testis. Samples were incubated with SPAG11A Polyclonal antibody (Product # PA5-116883) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-116883
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-50 of human SPAG11A (NP_4781082)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.86 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901513
Product Specific Information
Positive Samples: Rat testis
Immunogen sequence: MRQRLLPSVT SLLLVALLFP GSSQARHVNH SATEALGELR ERAPGQGTNG
Target Information
Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation. SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated. SPAG11A belongs to this family and is also named as EP2, HE2. It has 4 isoforms produced by alternative splicing.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antimicrobial peptide Bin1b; Antimicrobial-like protein Bin-1b; beta defensin EP2e; EP2 protein; sperm associated antigen 11; sperm-associated antigen 11; Sperm-associated antigen 11A
Gene Aliases: 9230111C08Rik; Bin1b; Ep2; EP2e; EP2Q; Spag11; Spag11a; Spag11b
UniProt ID: (Mouse) Q8K4N2, (Rat) Q8VBV2
Entrez Gene ID: (Mouse) 78128, (Rat) 246305
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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