The pPICZ A, B, & C Pichia Vectors are designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein. These vectors contain the Zeocin™ resistance gene for direct selection of multi-copy integrant strains. By selecting with increasing amounts of Zeocin™, strains with multiple copies of your gene of interest integrated into the genome are obtained. Increasing the number of copies of the gene of interest in a recombinant Pichia strain can result in higher expression levels. The vectors are included in the EasySelect™ Pichia Expression Kit (Cat. No. K1740-01).
Features of the pPICZ vectors include:
• Inducible AOX1 promoter for high-level expression in Pichia pastoris c-myc epitope tag for convenient detection with an Anti-myc Antibody • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody • Zeocin™ resistance for direct selection of multi-copy integrants
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
AOX1
Product Type
Pichia Expression Vector
Antibiotic Resistance Bacterial
Zeocin™ (ZeoR)
Protein Tag
His Tag (6x), c-Myc Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Quantity
20 μg
Vector
pPIC
Contents & Storage
20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.