pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid,Read more
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Catalog number V652020
also known as V6520-20
Price (EUR)/ 20 µg
1 160,00
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Add to cart
Price (EUR)/ 20 µg
1 160,00
Add to cart
pcDNA™5/FRT/TO Vector Kit
Catalog numberV652020
Price (EUR)/ 20 µg
1 160,00
-
Add to cart
pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.
The pcDNA™5⁄FRT⁄TO vector contains the following elements:
• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells • Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest • FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line • Hygromycin resistance gene for selection of stable cell lines
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemInducible
Delivery TypeTransfection
Inducing AgentTetracycline
PromoterCMV/TO
Product TypeFlp-In/T-REx System Expression Vector
Selection Agent (Eukaryotic)Hygromycin
Protein TagUntagged
Cloning MethodRestriction Enzyme/MCS
Quantity20 μg
VectorpcDNA
Product LineFlp-In™, T-REx™, pcDNA™
For Use With (Application)Regulated Expression
Unit Size20 µg
Contents & Storage
Contains 20 μg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored.