Nunc™ Cell Culture Inserts in Carrier Plate Systems
Nunc™ Cell Culture Inserts in Carrier Plate Systems
Thermo Scientific™

Nunc™ Cell Culture Inserts in Carrier Plate Systems

Utilisez jusqu’à 24 inserts simultanément grâce à la plaque de support Thermo Scientific™ Nunc™ pour inserts de culture cellulaire, utilisable pour des cultures en compartiment individuel ou des cultures partageant le même milieu.
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RéférencePorositéNbre de puits
1410828 μm12
1410020,4 μm24
1410043 μm24
1410068 μm24
141008(Sans insert)24
1410780,4 μm12
1410803 μm12
141086(Sans insert)12
Référence 141082
Prix (EUR)
421,00
Case of 4
Délai de disponibilité estimé  En attente  
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Porosité:
8 μm
Nbre de puits:
12
Prix (EUR)
421,00
Case of 4
Ajouter au panier
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Le système de plaque portoir Thermo Scientific™ Nunc™ est conçu pour permettre trois réglages de la hauteur différents pour les inserts de culture cellulaire afin d’accommoder une culture d’interface air-liquide et une plusieurs protocoles d’alimentation des cellules. Emballé individuellement, le système de plaque multipuits portoir est livré avec ou sans inserts de culture cellulaire.

Caractéristiques :
  • Chaque système comprend une plaque de culture cellulaire multipuits standard, une plaque de portoir et un couvercle. Le système est livré avec ou sans inserts de culture cellulaire
  • Pour régler la hauteur de suspension de l’insert dans le puits, faites pivoter l’insert pour engager l’une des trois languettes de réglage de la hauteur dans la plaque de portoir
  • Les plaques sont étiquetées avec une identification alphanumérique des puits
  • La membrane poreuse en polycarbonate de l’insert est traitée par culture cellulaire pour une fixation cellulaire optimisée
  • Les inserts sont disponibles en 3 tailles de pores : 0,4, 3 et 8 microns
  • Les plaques sont disponibles en deux formats : 12 et 24 puits
Garantie : 90 jours
Clause de non-responsabilité relative aux restrictions d’utilisation pour la recherche interne : toutes les restrictions qui s’appliquent à l’acheteur dans le cadre de l’utilisation du/des produit(s) à des fins de recherche interne uniquement ne s’appliquent pas au(x) produit(s) sur les pages Web associées à cette déclaration.
Spécifications
CouleurTransparent
Type de récipientSystèmes de plaques de support
Nbre par carton4
Nb par paquet1
Traitement de surfaceCulture cellulaire
Surface de culture1,13 cm2
À utiliser avecInsert de culture cellulaire
Inserts par plaque12
MatériauPC (membrane)
Nbre de puits12
Densité des pores< 0,85 x 10^5
Porosité8 μm
StérilitéStérile
TypeInsert de culture
Volume (métrique) utileSuspension basse : 2ml ; suspension moyenne : 3 ml ; suspension élevée : 4 ml
Unit SizeCase of 4
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Foire aux questions (FAQ)

What is the difference between the 24-well plate format for the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620) and the 24-well plate format for the Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002)?

The difference between the two 24-well plate formats is as follows:

  • Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620): There are only 12 inserts in the 24-well multi-well plate, leaving every second column free without an insert. This is a preferred configuration for some co-culture experiments, as it makes it easy to access the culture grown in the multi-well plate for media changes, etc., by placing the insert in the empty well next to it. The inserts are placed directly into the wells here, leaving a 0.9 mm gap between the bottom of the well and the insert membrane.
  • Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002): There are 24 inserts in the 24-well carrier plate. The carrier plate with the hanging slots allows for adjustment of the insert height. The three hanging positions in the carrier plate allow for distance of approximately 0.9 mm, 3.3 mm, or 6.3 mm between the well-bottom and insert growth surface for both 12- and 24-well plate formats. This configuration is preferred when customer needs more versatility, for example to facilitate air-liquid interface culture, and other co-culture applications.


Note: details on plate configuration can also be found within the following product brochure (pg 3).

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

What is the difference between your cell culture membrane inserts and carrier plates?

Standard culture membrane inserts are placed directly into the wells of a Multidish, and the feet at the bottom of the insert maintain a distance of about 1 mm between the membrane and the Multidish culture surface. The “carrier plate” culture membrane product adds a tray between the Multidish and the lid, with a set of hanger brackets at each well. These brackets engage one of three sets of tabs on each insert, to allow for selection between 3 different distances (approximately 1, 3, or 6 mm) between the membrane and the Multidish culture surface. This can allow for more flexibility in experiments, such as distance-dependent interaction between populations or media volume under cultures at the air-media interface.

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