Tube pour ARN sanguin Tempus™
Tube pour ARN sanguin Tempus™
Applied Biosystems™

Tube pour ARN sanguin Tempus™

Les tubes d’ARN de prélèvement sanguin Tempus sont destinés à la stabilisation et à l’isolement de l’ARN total à partirAfficher plus
Have Questions?
RéférenceQuantité
434279250 tubes
Référence 4342792
Prix (EUR)
471,65
Online Exclusive
524,00
Économisez 52,35 (10%)
Each
-
Ajouter au panier
Quantité:
50 tubes
Recurring order eligible. Learn more »
Prix (EUR)
471,65
Online Exclusive
524,00
Économisez 52,35 (10%)
Each
Ajouter au panier
Ask our AI about this Product
Les tubes d’ARN de prélèvement sanguin Tempus sont destinés à la stabilisation et à l’isolement de l’ARN total à partir du sang total pour l’analyse d’expression génétique. Avantages des tubes d’ARN de prélèvement sanguin Tempus :

• Lyser immédiatement les cellules de sang total et stabiliser l’ARN en une seule étape : ni lyse des érythrocytes ni traitement de protéinase K requis
• Congeler les profils globaux d’expression génétique pendant cinq jours maximum à température ambiante, et pendant plus de sept jours à 4°C

Fonctionnement
Chaque tube d’ARN de prélèvement sanguin Tempus contient 6 ml de réactif de stabilisation. Lorsque le sang (3 ml) est prélevé dans le tube et mélangé à ce réactif, la lyse se produit quasiment instantanément. Le réactif stabilisant désactive les RNase cellulaires et précipite de manière sélective l’ARN ; l’ADN génomique (ADNg) tandis que les protéines restent dans la solution. Le sang prélevé dans le tube d’ARN de prélèvement sanguin Tempus est stable pendant cinq jours maximum à température ambiante ou au moins sept jours à 4°C.

Produit accessoire
Lorsque les tubes d’ARN de prélèvement sanguin Tempus sont utilisés avec le kit d’isolement d’ARN par centrifugation Tempus (n° de réf. 4380204), des ARN à stabilité accrue et des rendements supérieurs d’ARN sont produits.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
DescriptionTubes d’ARN sanguin
Quantité50 tubes
Conditions d’expéditionTempérature ambiante
Quantité de matériel de démarrage3 mL of Whole Blood
Type d’échantillonWhole Blood
Unit SizeEach
Contenu et stockage
Stocker tous les tubes non utilisés à température ambiante.
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Figures

Documentation et téléchargements

Certificats

Numéro de lotCertificate TypeDateCatalog Number(s)
A2502368Certificate of Analysis27 juin 20254342792
A2412357Certificate of Analysis27 févr. 20254342792
A2412356Certificate of Analysis21 févr. 20254342792
A241036LCertificate of Analysis18 févr. 20254342792
A2309369Certificate of Analysis27 janv. 20254342792
5 résultats affichés, recherchez un certificat spécifique ci-dessus

Safety Data Sheets

Foire aux questions (FAQ)

No, please follow the RNA isolation methods described with the Tempus system. The collection tubes are not compatible with the RiboPure kit.

Our general recommendations are that once blood has been collected in the Tempus Blood RNA Tubes, you can store the tube at room temperature for up to 5 days or at 4 degrees C for up to 7 days. For long term storage, the tube can be frozen at -80 degrees C for at least 1.5 years.

We do not offer the Stabilizing Reagent that comes inside the Tempus Blood RNA Tube as a stand-alone item.

Equine whole blood has been successfully tested using this system, getting RINs around 9. Bovine blood should work similarly since there is not much of a difference between the two species for RNA isolation from whole blood, but this has not been tested. The protocol would be the same as the user guide and samples should be mixed vigorously immediately after blood is drawn into the tube for at least 10 secs, incorporating tube inversion to be sure the sample is stabilized.

The RNA stabilization solution in Tempus Blood RNA Tubes will not lyse E. coli cells at ambient temperature. If lysis of gram-negative bacteria is desired, mix the sample thoroughly with the RNA stabilization solution, and heat the lysate at 95 degrees C for 5 mins.

Citations et références (7)

Citations et références
Abstract
Assessment of sample collection and storage methods for multicenter immunologic research in children.
Authors:Matheson LA, Duong TT, Rosenberg AM, Yeung RS
Journal:J Immunol Methods
PubMed ID:18771669
'Multicenter studies involving both large and small centers separated by significant distances pose unique challenges to biological sample collection. The objective of this study was to evaluate protocols for determining inflammatory biomarkers that are cost and manpower efficient for handling blood destined for a sample repository. Tempus (Applied Biosystems) and ... More
Peripheral blood gene expression: it all boils down to the RNA collection tubes.
Authors:Menke A, Rex-Haffner M, Klengel T, Binder EB, Mehta D
Journal:BMC Res Notes
PubMed ID:22214347
'Gene expression profiling from peripheral blood is a valuable tool for biomarker discovery in clinical studies. Different whole blood RNA collection and processing methods are highly variable and might confound comparisons of results across studies. The main aim of the study was to compare genome-wide gene expression profiles obtained from ... More
High alpha-defensin levels in patients with systemic lupus erythematosus.
Authors:Sthoeger ZM, Bezalel S, Chapnik N, Asher I, Froy O
Journal:Immunology
PubMed ID:19191901
'Innate immunity plays a role in systemic lupus erythematosus (SLE). Our objective was to determine the levels of defensins, which are antimicrobial and immunomodulatory polypeptides, in SLE. Sera from SLE patients and healthy controls were tested for pro-inflammatory human beta-defensin 2 (hBD-2) and for alpha-defensin human neutrophil peptide 1 (HNP-1). ... More
CRY2 is associated with depression.
Authors:Lavebratt C, Sjöholm LK, Soronen P, Paunio T, Vawter MP, Bunney WE, Adolfsson R, Forsell Y, Wu JC, Kelsoe JR, Partonen T, Schalling M
Journal:PLoS One
PubMed ID:20195522
Abnormalities in the circadian clockwork often characterize patients with major depressive and bipolar disorders. Circadian clock genes are targets of interest in these patients. CRY2 is a circadian gene that participates in regulation of the evening oscillator. This is of interest in mood disorders where a lack of switch from ... More
Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation.
Authors:Asare AL, Kolchinsky SA, Gao Z, Wang R, Raddassi K, Bourcier K, Seyfert-Margolis V
Journal:BMC Genomics
PubMed ID:18847473
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. In both systems the blood is immediately lysed when collected into ... More
7 total citations

Partager la référence catalogue, le nom ou le lien.

1x1 image pixel for data collection