Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Référence | Quantité |
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4342792 | 50 tubes |
No, please follow the RNA isolation methods described with the Tempus system. The collection tubes are not compatible with the RiboPure kit.
Our general recommendations are that once blood has been collected in the Tempus Blood RNA Tubes, you can store the tube at room temperature for up to 5 days or at 4 degrees C for up to 7 days. For long term storage, the tube can be frozen at -80 degrees C for at least 1.5 years.
We do not offer the Stabilizing Reagent that comes inside the Tempus Blood RNA Tube as a stand-alone item.
Equine whole blood has been successfully tested using this system, getting RINs around 9. Bovine blood should work similarly since there is not much of a difference between the two species for RNA isolation from whole blood, but this has not been tested. The protocol would be the same as the user guide and samples should be mixed vigorously immediately after blood is drawn into the tube for at least 10 secs, incorporating tube inversion to be sure the sample is stabilized.
The RNA stabilization solution in Tempus Blood RNA Tubes will not lyse E. coli cells at ambient temperature. If lysis of gram-negative bacteria is desired, mix the sample thoroughly with the RNA stabilization solution, and heat the lysate at 95 degrees C for 5 mins.
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