Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium (A1517001), vitronectin (VTN-N) has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines. In addition, vitronectin (VTN-N) has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Guokai Chen et al. (1) in the laboratory of James Thomson, the VTN-N variant of vitronectin supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8™ Medium.
Vitronectin (VTN-N) is:
• Optimized—designed in the laboratory of James Thomson for use with Essential 8™ Medium • Defined—for reduced variability in your PSC cultures • Cost effective—for economical and scalable PSC culture
Optimized and Defined Surface Unlike standard basement membrane extracts (BMEs), vitronectin (VTN-N) was designed in the laboratory of James Thomson for use with Essential 8™ Medium and has been shown to support human pluripotent stem cell attachment and survival better than wild type vitronectin. Vitronectin (VTN-N) provides an optimal environment for feeder-free pluripotent stem cell culture.
Reduce Variability Vitronectin (VTN-N) is a defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs).
Cost Effective When used with Essential 8™ Medium, vitronectin (VTN-N) provides a cost effective, defined system for feeder free culture of human pluripotent stem cells (PSCs).
Commercialized in Partnership with Cellular Dynamics International.
For Research Use Only. Not for use in diagnostic procedures.
Spécifications
Concentration
500 μg⁄ml
À utiliser avec (application)
Stem Cell Culture
Méthode de purification
HPLC
Quantité
1 mL
Espèces
Human
Recombinant
Recombinant
Classification
AOF
Niveau d’endotoxines
Very Low
Conditions d’expédition
Dry ice
Volume (métrique)
1 mL
Forme protéique
Recombinant
Forme
Liquid
Sous-type de protéine
Vitronectin
Catégorie de recherche
Stem Cell Research
Pureté ou qualité
95 %
Contenu et stockage
Store at -80°C and protect from light.
Figures
Documentation et téléchargements
Certificats
Recherchez par numéro de lot ou numéro de lot partiel