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Application
FIGURE: 1 / 3
SMO Antibody (MA5-56875) in WB
Western Blot analysis of SMO using SMO Recombinant Monoclonal Antibody (Product # MA5-56875). Boiled and unboiled mouse brain extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SMO antibody diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Product Details
MA5-56875
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL2975
Immunogen
Recombinant fragment encompassing a sequence within the Intracellular domain of human Smoothened homolog. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
AB_3679901
Target Information
Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bent body; frizzled family member 11; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; seven transmembrane helix receptor; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor
Gene Aliases: bnb; E130215L21Rik; FZD11; Gx; SMO; SMOH; smoothened
UniProt ID: (Human) Q99835, (Mouse) P56726
Entrez Gene ID: (Human) 6608, (Mouse) 319757
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