Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Référence | Quantité |
---|---|
R37627 | 1 kit |
The first step is to bind all endogenous biotinylated entities with an unlabeled avidin. The free D-biotin is then added to bind to all biotin binding sites on this added avidin. The free biotin will be washed away before any other labels are added and all biotin binding sites will be occupied on the unlabeled avidin.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Mitochondria have endogenous biotin, which is being labeled by the streptavidin. You will need to block the endogenous biotin first, after you fix and permeabilize, but before you block for your primary antibody.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The only difference between the ReadyProbes Avidin/Biotin Blocking Solution (1X) and other endogenous biotin blocking kits is the ease of use. In the ReadyProbes Avidin/Biotin Blocking Solution (1X), we provide the two components already pre-mixed and ready to use.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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