Kit de solutions iBind™ Flex
Kit de solutions iBind™ Flex
Invitrogen™

Kit de solutions iBind™ Flex

Le kit de solution iBind Flex est destiné à être utilisé avec le dispositif iBind Flex Western, qui est unAfficher plus
Have Questions?
Modifier l'affichagebuttonViewtableView
RéférenceType de produitContenus des kits
SLF2020Kit de solutions FlexTampon iBind™ Flex 5X, Additif iBind™ Flex 100X
SLF2020X4Kit de solutions Flex
Référence SLF2020
Prix (EUR)
126,65
Online Exclusive
140,00
Économisez 13,35 (10%)
1 kit
Ajouter au panier
Type de produit:
Kit de solutions Flex
Contenus des kits:
Tampon iBind™ Flex 5X, Additif iBind™ Flex 100X
Prix (EUR)
126,65
Online Exclusive
140,00
Économisez 13,35 (10%)
1 kit
Ajouter au panier
Le kit de solution iBind Flex est destiné à être utilisé avec le dispositif iBind Flex Western, qui est un dispositif de traitement Western automatisé qui effectue chaque étape du flux de travail Western, du blocage aux lavages en passant par les incubations d’anticorps. Les solutions iBind agissent comme des solutions de blocage, de lavage et de dilution dans le processus d’immuno-détection iBind et ont été optimisées pour une viscosité idéale. Chaque solution est libérée des puits du dispositif iBind Flex vers la carte iBind Flex via un flux latéral séquentiel (SLF); les solutions sont ensuite déplacées vers la région d’empilement de la carte. La matrice en fibre de verre de la carte permet un flux homogène et cohérent des solutions vers la membrane, augmentant l’interaction antigène-anticorps.

En savoir plus sur les systèmes Western iBind ›

Le kit de solutions iBind Flex, associé au Dispositif iBind Flex Western et aux Cartes iBind Flex, apporte automatisation et simplicité au protocole Western Par rapport aux méthodes manuelles, le système iBind Flex Western nécessite moins d’anticorps primaires, permettant ainsi d’obtenir des résultats reproductibles. Le système est compatible avec les protocoles de détection en aval. Chaque kit de solution iBind Flex contient une solution suffisante pour 10 transferts midi ou 20 transferts mini. Pour les applications fluorescentes, utilisez le Kit de solution de détection fluorescente (FD) iBind Flex.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
À utiliser avec (équipement)Dispositif iBind Flex Western
Contenus des kitsTampon iBind™ Flex 5X, Additif iBind™ Flex 100X
Nbre de réactions10 transferts mini ou 20 transferts mini
Quantité1 kit
Conditions d’expéditionHomologué pour des expéditions à température ambiante ou sur glace mouillée
Méthode de détectionColorimétrique, chromogène, chimiluminescent
Membrane CompatibilityPVDF, nitrocellulose
Gamme de produitsiBind™
Type de produitKit de solutions Flex
Unit Size1 kit
Contenu et stockage
• Tampon IBind Flex 5X
• Additif iBind Flex 100Xconserver

Conserver entre 2 et 8°C.
iBlot 3 Western Blot Transfer System

Introducing iBlot 3 Western Blot Transfer System

Featuring higher throughput and built-in cooling for consistent protein transfer
Learn more

Foire aux questions (FAQ)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Documentation et téléchargements

Certificats

Safety Data Sheets

Partager la référence catalogue, le nom ou le lien.

1x1 image pixel for data collection