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Catalog Number | Unit Size | Price (HKD) | Availability | Quantity | |
---|---|---|---|---|---|
11352D | 2 mL | Price: 2,887.00 Your price: | *** | ||
11346D | 10 mL | Price: 8,957.00 Your price: | *** |
Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns required
Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Starting Sample:
PBMC