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7900HT Fast Real-Time PCR System with Fast 96-Well Block Module
7900HT Fast Real-Time PCR System with Fast 96-Well Block Module
Applied Biosystems™

7900HT Fast Real-Time PCR System with Fast 96-Well Block Module

The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility andRead more
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43514051 instrument
Catalog number 4351405
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The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan™ Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate.
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.
• 96- or 384-well plate compatibility (including the TaqMan™ Low Density Array).
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security.
• Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity.
• Proven assay development guidelines save time and money

High Throughput
The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An Automation Accessory combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.

Flexibility
The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats let you select the format that’s right for your project, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan™ Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets you add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.

Powerful Software
Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. You can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, you can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibrated DyeSYBR™ Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™
Detection MethodSYBR, Primer-Probe Detection
Dimensions72 cm (W) x 84 cm (D) x 64 cm (H) (with Drawer Open)
Dynamic RangeUp to 9 Logs of Linear Dynamic Range
For Use With (Equipment)7900HT Fast System
Format96-well Plate
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
OpticsExtended-life 488 nm Argon-ion Laser Excitation Source, No Filters (CCD acts as spectrograph with continuous detection 500 to 650 nm)
Passive Reference DyeROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
Quantity1 instrument
Reaction SpeedFast
Reaction Volume Range10-30 μl
Run Time33 min. (96-well in Fast Mode)
Sample Ramp RateFast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
SensitivityDown to 10 Copies
Temperature Range (Metric)4 to 100°C
Thermal Accuracy±0.25°C of Display Temperature
Thermal Cycling SystemPeltier-based System
Thermal Uniformity±0.5°C
Throughput≥5,000 Real Time PCR rxns per day (384-well block and automation accessory), ≥10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
Weight114 kg with Automation Accessory, 82 kg without Automation Accessory
Block FormatInterchangeable
CapacityUp to 84 x 384-well Plates (with optional Automation Accessory and 384-well Block), Up to 36 x 96-well Plates with optional Automation Accessory
Unit SizeEach
Contents & Storage
1 Instrument with Fast 96-well Block Module
Have questions about this product? Ask our AI assisted search.
I purchase my Applied Biosystems 7900HT Fast Real-Time PCR System. Can I still order user-interchangeable standard 96-well, 384-well, and TaqManLow Density Array blocks?
How long can the Applied Biosystems 7900HT Fast Real-Time PCR System run unattended, and how many plates can be processed in that time frame?
When doing routine maintenance on the Applied Biosystems 7900HT Fast Real-Time PCR System, I have noticed that in the background plate there are some wells that are above 2500 fluorescent intensity units. What does this mean?
I have optimized my assay on the ABI PRISM 7900HT SDS. Will I need to re-optimize my assay to run on a standard block on the Applied Biosystems 7900HT Fast Real-Time PCR System?
Is the Applied Biosystems 7900HT Fast Real-Time PCR System available with a standard 96-well or 384-well block?
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Limited Use Label Licenses (LULL)

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Notice to Purchaser: The purchase of this instrument conveys to the purchaser the limited, non-transferable right to use the purchased instrument only, under intellectual property rights that are owned and/or controlled by Life Technologies and relate specifically to the instrument. Purchase of the instrument includes the right to use the instrument for internal research and to perform services (including the right to report the results of services for a fee) by the purchaser only, but does not convey rights to use any other products, reagents, assays or methods such as the 5’ nuclease assay process. The sale of this instrument is expressly conditioned on the purchaser not reselling, repackaging, or distributing this instrument, or any of its components, and no such rights are conveyed expressly, by implication, or by estoppel. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Frequently asked questions (FAQs)

The threshold is the numerical value assigned for each run that reflects the average dRn (change in fluorescence) during the initial cycles of PCR (baseline). The threshold is set by determining a statistically significant point (or value) above the baseline. You can manually adjust the threshold should you desire, to the place in the geometric phase where your replicates are tightest. For more information on how to manually set a threshold, please refer to the tutorial entitled Data Analysis on the ABI PRISM 7700: Setting Baselines and Thresholds (P/N 4370923). While this tutorial is based on the ABI PRISM 7700 system, the concepts are still valid for the Applied Biosystems real-time PCR instruments. You can search the document on our website by using the part number above as the keyword.

If you choose not to manually adjust the baselines and thresholds, the SDS software on the Applied Biosystems real-time PCR instruments has an Auto Ct algorithm that can be used to automatically generate baseline and threshold values for individual detectors. The algorithm calculates baseline and threshold parameters for a detector based on the assumption that the data exhibits the "typical" amplification curve. Experimental error (i.e. contamination, pipetting inaccuracies) can produce amplification curves that deviate significantly from a typical amplification curve. The data from these irregularities can affect the Auto Ct algorithm by causing it to generate incorrect baseline and threshold parameters for the associated detector. Therefore, Applied Biosystems recommends that after analysis of experimental data, you review all baseline and threshold parameters determined by the Auto Ct algorithm.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

You have to run a calibration when a new sample block format is used in the 7900HT Fast Real-Time PCR System, but it is not necessary to run the pure dye calibration if the block has been used before. For example, if you use a 384-well sample block for the first time, then you have to run a 384-well block calibration. Later, should you switch to a 96-well block and then switch back to a 384-well block again, then there is no need to rerun the calibration of the 384-well sample block. We recommend that you run a background run each time you exchange the sample block because dust particles or other contaminants could get into the wells while the block is being stored outside of the instrument. Please consult the 7900HT User Manual, Cat. No. 4317596, for the recommended maintenance procedures.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The log file tracks events during the course of an instrument run. To locate the log file, open up the hard drive of the instrument computer. Open the Applied Biosystems folder, then the SDS 2.x.x folder, then the log folder. The log file is a Microsoft Word file in .doc format and is saved as the date the run was initiated.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

To program a long-term hold on the Applied Biosystems 7900HT Fast Real-Time PCR System, launch the SDS software and open a new plate. Click on the Instrument tab. Move the cursor to the end of the thermal cycling profile, below the time box, and click. This will create a solid vertical black line. Click on “Add Hold”. Change the temperature to 4.0 and the time to the highest possible time, 98:59. Although an infinity symbol will not appear, the instrument will run for 98 hours and 59 seconds. The run must be aborted when you want to analyze the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

While the Applied Biosystems 7900HT Fast Real-Time PCR System is idle, it puts off 6600 BTU/hour. While it is cycling, it puts off 8800 BTU/hour.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Citations & References (4396)

Citations & References
Abstract
Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.
Authors:Wong H; Lehman-McKeeman LD; Grubb MF; Grossman SJ; Bhaskaran VM; Solon EG; Shen HS; Gerson RJ; Car BD; Zhao B; Gemzik B
Journal:Toxicological Sciences : An Official Journal of the Society of Toxicology
PubMed ID:
4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyrimidine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell adenoma. The ... More
RAGE ligand upregulation of VEGF secretion in ARPE-19 cells.
Authors:Ma W; Lee SE; Guo J; Qu W; Hudson BI; Schmidt AM; Barile GR
Journal:Investigative Ophthalmology & Visual Science
PubMed ID:
PURPOSE: The importance of VEGF in stimulating neovascular age-related macular degeneration (AMD) is well-recognized, but the initiating factors that induce local upregulation of VEGF remain unclear. The current study was conducted to test the hypothesis that activation of RAGE (receptor for advanced glycation end products [AGEs]) by its ligands, including ... More
SEROTONIN POLYMORPHISMS AND POSTTRAUMATIC STRESS DISORDER IN A TRAUMA EXPOSED AFRICAN AMERICAN POPULATION
Authors:Mellman, TA; Alim, T; Brown, DD; Gorodetsky, E; Buzas, B; Lawson, WB; Goldman, D; Charney, DS
Journal:DEPRESSION AND ANXIETY
PubMed ID:
Background: Genetic polymorphisms that influence serotonin (5-hydroxytryptamine, 5HT) neurotransmission are candidates for contributing to susceptibility to posttraumatic stress disorder (PTSD). The objective of our study was to determine if a variable length polymorphism for the promoter regions of the 5HT transporter (5HTTLPR), and/or a substitution polymorphism. in the promoter region ... More
Cognitive Flexibility is Associated with KIBRA Variant and Modulated by Recent Tobacco Use
Authors:Zhang, HP; Kranzler, HR; Poling, J; Gruen, JR; Gelernter, J
Journal:NEUROPSYCHOPHARMACOLOGY
PubMed ID:
The kidney and brain expressed protein gene (KIBRA) and the calsyntenin 2 gene (CLSTN2) are reportedly involved in synaptic plasticity. Single nucleotide polymorphisms (SNPs) rs17070145 (KIBRA) and rs6439886 (CLSTN2) have been found to affect memory performance measures. This study examined the association of KIBRA SNP rs17070145 and CLSTN2 SNPs rs6439886 ... More
Phenomic determinants of genomic variation in autism spectrum disorders
Authors:Qiao, Y; Riendeau, N; Koochek, M; Liu, X; Harvard, C; Hildebrand, MJ; Holden, JJA; Rajcan-Separovic, E; Lewis, MES
Journal:Journal of Medical Genetics
PubMed ID:
Background: Autism spectrum disorders (ASDs) are common, heritable neurobiologic conditions of unknown aetiology confounded by significant clinical and genetic heterogeneity. Methods: This study evaluated a broad categorisation of phenotypic traits (or phenome) for 100 subjects with Autism Diagnostic Interview-Revised/Autism Diagnostic Observation Schedule-Generic (ADI-R/ADOS-G) confirmed idiopathic ASD undergoing 1 Mb ... More
4396 total citations

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