CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

The CaptureSelect AAT-XL Affinity Matrix in this RoboColumn has been specifically designed for the purification of native human alpha-1 antitrypsin (AAT) from complex sources like plasma/serum and recombinant human AAT from cell culture supernatants. The high selectivity and yield obtained using CaptureSelect AAT-XL Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in a single step.

Features of this affinity matrix include:

• Highly selective for native and recombinant human AAT
• High recovery and yield obtained in a single purification step
• Mild elution at pH 6
• Excellent scalability
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

• Column size: 200 μL, 1 strip, 8 columns (8-row)
• Column dimension: 5-mm diameter, 10-mm bed height
• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect AAT-XL affinity ligand
• Ligand coupling: epoxide coupling
• Binding capacity: 15 mg AAT/mL resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM Tris, 2.0 M MgCl₂, pH 6.0
• Formulation buffer: 20% (v/v) ethanol
• Operating pressure: 2 bar (maximum)
• Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.