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Rockland

p35 Polyclonal Antibody

View all (10) p35 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

p35 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 3

p35 Antibody (600-401-ME9S) in IHC

Immunohistochemistry of Rabbit Anti-p35 Antibody. Tissue: Mouse Brain. Fixative: none. Antigen Retrieval: HIER. Primary Antibody: Anti-p35/Cdk5 Antibody at 1:100. Secondary Antibody: Ready-to-Use Anti-Rabbit. Counterstain: Hematoxylin. Results: p35 developed a strong nuclear and membranous staining of neurons and neutrophil in brain tissue.

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Product Details

600-401-ME9S

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

1:100

ELISA (ELISA)

1:10,000-1:50,000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-p35/Cdk5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse p35/Cdk5 conjugated to Keyhole Limpet Hemocyanin (KLH).

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Conjugate

Unconjugated

Form

Liquid

Concentration

0.96 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against mouse p35/Cdk5. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat,human, bovine, and Spermophilus citellus.

Target Information

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDK5 activator 1; Cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; cyclin-dependent kinase 5, regulatory subunit (p35) 1; cyclin-dependent kinase 5, regulatory subunit 1 (p35); neuronal CDK5 activator; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; TPKII regulatory subunit

Gene Aliases: CDK5P35; CDK5R; CDK5R1; D11Bwg0379e; NCK5A; p23; p25; p35; p35nck5a

UniProt ID: (Human) Q15078, (Mouse) P61809

Entrez Gene ID: (Human) 8851, (Mouse) 12569

Function(s)

protein kinase activity cyclin-dependent protein serine/threonine kinase activity calcium ion binding protein binding kinase activity cyclin-dependent protein kinase 5 activator activity protein kinase binding protein serine/threonine kinase activator activity cadherin binding ephrin receptor binding cytoskeletal protein binding kinase activator

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity neuron migration neuron cell-cell adhesion G-protein coupled acetylcholine receptor signaling pathway axon guidance axonal fasciculation brain development cell proliferation embryo development regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cerebellum development superior olivary nucleus maturation hippocampus development layer formation in cerebral cortex neuron differentiation negative regulation of axon extension neuron projection development regulation of actin cytoskeleton organization serine phosphorylation of STAT3 protein ionotropic glutamate receptor signaling pathway positive regulation of neuron apoptotic process regulation of neuron differentiation negative regulation of transcription, DNA-templated ephrin receptor signaling pathway rhythmic process regulation of dendritic spine morphogenesis regulation of microtubule cytoskeleton organization positive regulation of cell cycle arrest positive regulation of protein serine/threonine kinase activity positive regulation of protein targeting to membrane regulation of signal transduction by p53 class mediator protein phosphorylation cerebral cortex radially oriented cell migration positive regulation of protein kinase activity regulation of cell cycle

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