Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Catalog Number | Unit Size | Price (HKD) | Availability | Quantity | |
---|---|---|---|---|---|
62247 | 10 mg | Price: 1,881.00 Your price: | *** |
Thermo Scientific Pierce DAPI Nuclear Counterstains are high-purity forms of diamidino-2-phenylindole dye for fixed-cell, fluorescent staining of DNA content and nuclei for cellular imaging techniques.
DAPI (diamidino-2-phenylindole) is a blue fluorescent probe that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA, where its fluorescence is approximately 20-fold greater than in the nonbound state. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content.
Features of DAPI Nuclear Counterstain:
Properties of DAPI Fluorescent Dye:
As a counterstain in fluorescence imaging methods, DAPI is compatible with antibodies and other probes labeled with fluorescein (Cat. No. 53027) and rhodamine (Cat. No. 46112) dyes, as well as with Thermo Scientific DyLight Fluors. DAPI has greater photostability than Hoechst dyes, although Hoechst 33342 can be use for live cell imaging while use of DAPI is confined to fixed cells. DAPI is offered in powdered solid and aqueous solution forms.
Applications for DAPI Stain:
Related Products