Pierce™ Anti-HA Magnetic Beads
Pierce™ Anti-HA Magnetic Beads
Thermo Scientific™

Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
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Catalog NumberQuantity
888361 mL
888375 mL
Catalog number 88836
Price (HKD)
3,347.00
Each
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Quantity:
1 mL
Request bulk or custom format
Price (HKD)
3,347.00
Each
Add to cart
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Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-HA Magnetic Beads:

  • Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation
  • Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
  • Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments
  • Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Specifications:

Product Details:

Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.

For Research Use Only. Not for use in diagnostic procedures.
Thermo Scientific™ Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA–tagged proteins and the co–immunoprecipitation (co–IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA–tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50mM NaOH, or SDS–PAGE sample buffer. If gentler elution conditions are desired, 2mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA–tagged protein by Western blot analysis.
Specifications
ConcentrationSlurry: 10mg/mL, 1% solids
Ligand TypeAntibody
Protein FormRecombinant
Quantity1 mL
TargetHA
Capacity (Metric)1 mL
FormLiquid
Particle Size1 μm
Product LinePierce™
TypeMagnetic Affinity Bead
Unit SizeEach
Contents & Storage
Store at 4°C.
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