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The BacMam Histone H3 Reagent provides a convenient genetic delivery tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. The GFP fusion enables sensitive detection of post-translational modifications using LanthaScreen™ TR-FRET Cellular Assay technology.
The BacMam Histone H3 Reagent Lets You:
Get More Physiologically Relevant Results
The BacMam Histone H3 allows the investigation of post-translational modifications of histone H3 in your choice of cellular backgrounds, including primary cells. This enables screening for potential inhibitors of histone-modifying enzymes such as kinases, acetylases, deacetylases, methyl transferases, and demethylases in their natural complexes in a physiologically relevant cell type.
BacMam and LanthaScreen™ Convenience Saves Sample and Time
When used with a LanthaScreen™ Tb-anti-Histone H3 antibody against a specific modification, assays can be run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA. By using the same BacMam reagent for GFP-Histone H3, a variety of histone H3 modifications (Ph, Me, Ac) can be monitored just by exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen™ technology includes all of the advantages of TR-FRET detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells.