Perfringens TSC Agar Base (Dehydrated)

Selectively isolate and presumptively identify Clostridium perfringens in food samples with Thermo Scientific™ Oxoid™ Perfringens TSC Agar Base (Dehydrated). The medium can either be used on its own or with selective agents to make Tryptose Sulphite (TS) Agar, Tryptose Sulphite Cycloserine (TSC)¹ agar or Shahadi Ferguson Perfringens (SPF)² agar for the presumptive identification and enumeration of Clostridium perfringens.

Meat peptone, soy peptone and yeast extracts provide essential nutrients and vitamins for the development of clostridia. Sodium metabisulphite and ferric ammonium citrate act as an indicator of sulphide reduction, indicated by black coloured colonies.

Some strains of Clostridium perfringens may produce an opaque zone around the colony due to lecithinase activity, but this is not considered to be universal for all Clostridium perfringens strains after overnight incubation³, and both black, lecithinase positive and black, lecithinase negative colonies should be considered as presumptive Clostridium perfringens on TSC Agar and confirmatory tests carried out.

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