GeneBLAzer™ NPSR1-A CHO-K1 DA Assay Kit

The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the nuclear factor of activated T-cell (NFAT) response element.

The NPSR1-A CHO-K1 DA cells and NPSR1-A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using Neuropeptide S. In addition, NPSR1-A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.