Tango™ HTR2A-bla U2OS Cells
Product Image
Thermo Scientific™

Tango™ HTR2A-bla U2OS Cells

The Tango™ HTR2A-bla U2OS cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and  Read more
Have Questions?
Catalog number K1562
Price (HKD)/ 8 x 106 cells
-

The Tango™ HTR2A-bla U2OS cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HTR2A-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR2A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNM_000621.2
Assay EntryCell-based beta-arrestin recruitment
Cell StateDividing Cells
Cytoplasmic TailTail from AVPR2 GPCR
Detection MethodFluorescence
Gene SymbolHTR2A
Population StatusSingle Cell Clone Origin
Product LineGeneBLAzer™
Protein FamilyGPCR (G-Protein Coupled Receptors)
Quantity8 x 106 Cells
ReadoutEnd Point
Reporter GeneBLA (Beta-Lactamase)
Target EntryHTR2A
Cell LineU2OS
System TypeTango™
Druggable TargetGPCR
Unit Size8 x 106 cells
Contents & Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ HTR2A-bla U2OS cells
• Tango™ HTR2A-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ HTR2A-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.