TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells
Invitrogen™
TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells
TOPO™ TA Cloning™ Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include theRead more
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Catalog Number
Quantity
K4600J10
10 Reactions
K460001
25 Reactions
K460040
50 Reactions
Catalog number K4600J10
Price (HKD)/ 10 reactions
Price:3,335.00
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Quantity:
10 Reactions
TOPO™ TA Cloning™ Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO™ TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the most reliable cloning method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants.
Convenient features of this TOPO™ TA Cloning™ Dual Promoter kit include: • 3´-T overhangs for direct ligation of Taq-amplified PCR products • T7 and SP6 promoters for efficient in vitro transcription • M13 forward and reverse primer sites for sequencing • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning • Kanamycin and ampicillin resistance for your choice of selection in E. coli • Easy blue/white colony screening for selection of recombinants • Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies
Kit Options: TOPO™ TA Cloning™ Dual Promoter Kits TOPO™ TA Cloning™ Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs: • General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40) • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40) • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40) • Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20) • Repressor/induction needs: TOP10F’ cells (Cat. Nos. K4650-01, K4650-40) • Provide your own cells (Cat. Nos. 451641, 450641)
For Research Use Only. Not for use in diagnostic procedures.