Exosome-Human CD81 Flow Detection Reagent (from cell culture) enables detection of CD81-positive extracellular vesicles (ECVs) such as exosomes in enriched cell culture media using flow cytometry or electron microscopy. Free exosomes alone are too small to be detected by most flow cytometry instruments. By employing Dynabeads™ magnetic separation technology, exosomes can be easily visualized by flow cytometry while bound to the bead surface. The 2.7 μm Dynabeads™ magnetic beads allow for a clear and defined flow analysis.

• Minimal hands-on time for specific exosome capture and analysis

• Clear and defined flow analysis in less than 1 hour hands-on time

• Study expression of specific exosome subset markers

• Protocol is easily scalable

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads™ magnetic separation technology, these subsets can be selectively captured and studied using flow cytometry and/or electron microscopy. And with Dynabeads™ technology you avoid losing material due to the handling and centrifugation typically experienced with other isolation and analysis methods.

The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.