Defined Keratinocyte SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers. The growth-promoting activity of BPE has been replaced by the inclusion of growth-promoting agents, which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM), allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.
Defined Keratinocyte SFM is a complete serum free medium consisting of Defined Keratinocyte Serum Free Basal Medium with the addition of Defined Keratinocyte SFM Growth Supplement that eliminates the requirement for bovine pituitary extract. The supplement is supplied in separate packaging with the basal medium.
For Research Use Only. Not for use in diagnostic procedures.
規格
Cell Type
Keratinocyte Cells, Epithelial Cells
Classification
Serum-free
Concentration
1 X
Culture Type
Mammalian Cell Culture
Form
Liquid
Product Type
Kit
Serum Level
Serum-free
Shelf Life
12 Months
Sterility
Sterile-filtered
Sterilization Method
Sterile-filtered
With Additives
Glutamine, Phenol Red
Green Features
Sustainable packaging
Quantity
500 mL
Unit Size
500 mL
內容物與存放
• 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light • 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5 to -20°C) and protect from light
Complete Medium: store in refrigerator (2–8°C) and protect from light.