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Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.
Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)
Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™
Starting sample
PBMC
For Research Use Only. Not for use in diagnostic procedures.
規格
Cell Type
T Cells (Regulatory)
Product Type
Cell Isolation Kit
Isolation Technology
Depletion, then positive isolation
No. of Cells
Processes ∼1 x 109 cells total
Output Viability
>95%
Product Line
DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
Purity or Quality Grade
Research Grade
Sample Type
PBMC
Shipping Condition
Room Temperature
Starting Material Cell No.
1 x 108 PBMCs per isolation
Target Species
Human
內容物與存放
Each Kit Contains:
• 2 mL Antibody Mix Human CD4
• 10 mL Depletion MyOne™ Dynabeads™
• 5 mL Dynabeads™ CD25
• 2 mL DETACHaBEAD™.
These reagents are sufficient to process ∼1 x 109 cells. Store components at 2°C to 8°C.
• 2 mL Antibody Mix Human CD4
• 10 mL Depletion MyOne™ Dynabeads™
• 5 mL Dynabeads™ CD25
• 2 mL DETACHaBEAD™.
These reagents are sufficient to process ∼1 x 109 cells. Store components at 2°C to 8°C.
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