Thermo Scientific™ Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.
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產品號碼
Description
Detection Method
15442
Clear, 96-well
Colorimetric
15342
Black, 96-well
Fluorescence
15142
Clear, 8-well Strip
Colorimetric
15242
White, 96-well
Chemiluminescence
產品號碼 15442
價格 (HKD)/ 5 plates
價格2,712.00
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新增至購物車
Description:
Clear, 96-well
Detection Method:
Colorimetric
Request bulk or custom format
價格 (HKD)/ 5 plates
價格2,712.00
新增至購物車
Pierce™ Nickel Coated Plates
產品號碼15442
價格 (HKD)/ 5 plates
價格2,712.00
***
新增至購物車
These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.
Features of Pierce Nickel Coated Plates include: • Ni2+ activated surface enables metal-chelate binding of His-tagged proteins • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene • Better binding for sensitive assays compared to other commercially available nickel-activated plates • Detection limit: 1 ng of polyhistidine fusion protein • Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well • Activation level: 200 μL
These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.
For Research Use Only. Not for use in diagnostic procedures.
規格
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Packaging5 Plates
Product LinePierce
ColorClear
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
內容物與存放
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.