Collagenase, Type I, powder
Collagenase, Type I, powder
Gibco™

Collagenase, Type I, powder

Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro,深入閱讀
Have Questions?
變更視圖buttonViewtableView
產品號碼Quantity
171000171 g
17018029500 mg
產品號碼 17100017
價格 (HKD)
2,808.00
Each
新增至購物車
Quantity:
1 g
價格 (HKD)
2,808.00
Each
新增至購物車
Ask our AI about this Product
Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.

Gibco™ Collagenase Type I is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and organ perfusions. Gibco™ Collagenase Type I activity is guaranteed to be greater than 125 units/mg. Compared to other collagenase preparations, Gibco™ Collagenase Type I has average levels of collagenase, caseinase, clostripain, and tryptic activities, and is well-suited for the digestion of fat, adrenal, and liver cells or tissues.
For Research Use Only. Not for use in diagnostic procedures.
規格
Quantity1 g
Shelf Life24 Months
Shipping ConditionRoom Temperature
FormLyophilized
Product TypeCollagenase
SterilityNon-sterile
Unit SizeEach
內容物與存放
Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

常見問答集 (常見問題)

Can collagenase be used to dissociate feeder free hESC when cultured on CELLstart medium?

Yes, collagenase can be used. It is recommended to use a 10 mg/ml collagenase IV solution (0.5 ml per 6 cm dish). After 1 minute, remove collagenase and rinse cells. Scrape cells into wash buffer (see product manual for more information), pellet, and re-plate as desired.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I make the 1000X stock (100 U/µL) solution from Collagenase powder?

1. Add 1 mL Hanks' Balanced Salt Solution (HBSS) with calcium and magnesium directly to 1 g vial of Collagenase. Vortex gently to ensure complete dissolution. Transfer to a clean tube.
2. Determine volume of HBSS (with calcium and magnesium) required to bring collagenase solution to 100 U/µL (1000X stock solution). The activity is lot- specific. Rinse vial with this volume of HBSS (with calcium and magnesium), and combine. Filter sterilize 1000X stock solution with a low protein binding filtration unit.
Example: Assuming the lot you have purchased has an activity of 265 U/mg, this lot will have 265000 Units per mL when you reconstitute collagenase into HBSS (with calcium and magnesium) at 1 g/mL. In order to dilute 265000 U/L to 100000 U/mL (= 100 U/µL), you need to dilute the 1 g/mL enzyme solution 2.65 fold.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

文件與下載

證書

安全資料表

分享目錄編號、產品名稱或連結。

1x1 image pixel for data collection