The Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqMan MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
This is a highly specific kit that quantitates only mature miRNAs, not precursors and a sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is a fast, simple, and scalable two-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Note: The amount of RT enzyme included in the kit is sufficient for single tubes but not for arrays.
For Research Use Only. Not for use in diagnostic procedures.
規格
Detection Method
Primer-probe
GC-Rich PCR Performance
High
PCR Method
2-step RT-qPCR
Reaction Speed
Fast
Optimal Reaction Temperature
42°C
Product Line
TaqMan™
Product Type
MicroRNA Reverse Transcription Kit
Reverse Transcriptase
MultiScribe™
Shipping Condition
Dry Ice
Sufficient For
1000 Reactions
For Use With (Application)
miRNA analysis
Concentration
10X
Fidelity (vs. Taq)
10 X
Final Product Type
cDNA
No. of Reactions
1000 Reactions
Reaction Format
Separate Components
Sample Type
RNA
Size (Final Product)
200 bp or less
內容物與存放
Contains 10X RT buffer, dNTP mix w/dTTp (100 mM total), RNase inhibitor (20 U/μL), and MultiScribe™ RT enzyme (50 U/μL) for 1000 reactions. Store at -15 to -25°C.