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The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen® Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Thr308.
For Research Use Only. Not for use in diagnostic procedures.
規格
Assay Entry
Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient)
Cell State
Cells Not Included
Detection Method
Fluorescent
For Use With (Application)
TR-FRET
Human Protein Class
Kinases, Cell Signalling
Product Line
LanthaScreen™
Readout
End Point
Reporter Gene
GFP (EmGFP)
Target Entry
AKT1
System Type
LanthaScreen™
Druggable Target
Kinases
內容物與存放
Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation.
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