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The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (less than 1 EU/μg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.
Advantages of the PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
• High yields—isolate up to 0.4 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.
Advantages of the PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
• High yields—isolate up to 0.4 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
For Research Use Only. Not for use in diagnostic procedures.
規格
Format
Kit
Isolation Technology
Silica Column
Scale
Midi
For Use With (Application)
Cloning, PCR
No. of Reactions
50 Preps
Prep Scale
Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg
Product Line
PureLink™
Product Type
Plasmid Purification Kit
Quantity
50 Preps
Shipping Condition
Room Temperature
Test Time
30 min.
內容物與存放
• Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature
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