The Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit allows simultaneous gene expression measurement of over 20,000 mouse RefSeq genes in a single assay. Providing the ability to work directly with low input levels of total RNA, the Ion AmpliSeq Transcriptome Mouse Gene Expression Assay is ideal for those interested in obtaining comprehensive and highly sensitive gene expression measurements from a wide range of RNA sources. This particular kit enables automated production of Ion AmpliSeq transcriptome libraries on the Ion Chef System. For manual production, see the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit.

The Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit offers:
• Flexible, straightforward library preparation—start with as little as 10 ng of total RNA from tissues, cells, or other sources
• Fast gene expression profiling with simple workflow—go from RNA to data in <2 days with ∼45 minutes of total hands-on time with the automated workflow of the Ion GeneStudio S5 and Ion Chef systems
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting as well as quality control metrics
• Compatibility with Ion Xpress Barcode adapters, allowing for multiplexed sequencing

Leveraging the speed and simplicity of Ion AmpliSeq technology, this ultrahigh-multiplex PCR enrichment approach enables rapid gene expression profiling with minimal hands-on time. This research method provides a faster, simpler, and lower-cost sequencing method that is highly correlated to whole-transcriptome RNA sequencing.

Straightforward library preparation and multiplex sequencing with Chef-Ready Kit
The Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit, includes the reagents and materials required for automated preparation of 32 barcoded Ion AmpliSeq transcriptome libraries. The Ion Chef System can automate up to eight barcoded libraries per run, so the kit provides reagents and materials sufficient for four runs. Starting with as little as 10 ng of total RNA, the total RNA is first processed using the SuperScript VILO cDNA Synthesis Kit (not included), and the resulting cDNA amplified using Ion AmpliSeq technology to accurately maintain expression levels of all genes. Approximately 7 hours of instrument run time and only 15 minutes of hands-on time are needed to set up the Ion Chef System for library construction from cDNA.

The resulting libraries are ready for downstream template preparation using the Ion Chef System and sequencing on the Ion GeneStudio S5 or Ion Proton sequencing system. With the recommended 16 libraries per Ion 550 Chip and 8 libraries per Ion 540 or Ion PI chip, libraries are multiplexed for flexibility, throughput increase, and cost reduction. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.