SureCount™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit

Rapidly and simultaneously detect, quantify, and differentiate Salmonella species, Salmonella Typhimurium, and Salmonella Enteritidis in a single real-time PCR test with the Thermo Scientific™ SureCount™ Salmonella species, Typhimurium and Enteritidis Multiplex PCR Kit. Replace labor and time-consuming traditional enumeration methods with SureCount™ rapid workflows for meat, poultry, and pork matrices. Understand Salmonella intervention effectiveness and make risk-based food safety decisions with fully automated Salmonella quantification and serotype differentiation results in as little as 8 hours using the Thermo Scientific™ RapidFinder™ Analysis Software version 3.0 or later. The kit is compatible with the Applied Biosystems™ Quant Studio™ 5 Food Safety PCR System.

Increase workflow efficiency and gain greater insight into Salmonella control across the production chain by replacing traditional enumeration and strain typing methods with the SureCount™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR method. The real-time PCR kit enables rapid, simultaneous detection and quantitation of Salmonella species, Salmonella Typhimurium, and Salmonella Enteritidis in a single PCR reaction, with workflows for a wide variety of matices including poultry, beef, and pork matrices including  poultry and meat finished goods workflows (AOAC Performance Tested Methods^SM certified), and custom protocols available for in-process carcass rinses and primary production samples.

• Real-Time Decisions: Improve process control and product safety by obtaining Salmonella quantification and serotype results in a single shift
• Cost and Labor Savings: Replace labor and consumable intensive MPN methods with quantitation in a single PCR reaction. Save on the cost of further analysis methods to differentiate Salmonella
• Confident Results: Applied Biosystems™ TaqMan™ Real-Time PCR probes provide a high level of specificity and sensitivity for industry-relevant serovars. Internal positive controls give added confidence
• Automated Results: RapidFinder Analysis Software v3.0 or later automatically reports Salmonella serovars and quantitation results

SureCount™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit includes:

• Pre-dispensed and ready-to-use reagents for cell lysis and release of DNA from bacteria present in enriched food and environmental samples
• Pre-dispensed lyophilized PCR pellets containing target-specific primers, probes, Internal Positive Control (IPC), and reagents for multiplex real-time PCR using the prepared lysate
• According to the AOAC-PTM validation study, the method is able to quantify 1 CFU per tested poultry rinses, raw meat, and poultry samples: chicken carcass rinses, ground beef, ground pork, and ground turkey