Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
產品號碼 | 包裝規格 | 價格 (HKD) | 供應狀況 | 數量 | |
---|---|---|---|---|---|
AM8439 | 50 µl | 價格 1,905.00 您的價格 | *** |
When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.