Primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture. • Viability at least深入閱讀
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產品號碼
Quantity
C0045C
1 vial
產品號碼 C0045C
價格 (HKD)
-
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Quantity:
1 vial
Primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture. • Viability at least 70% • ≥500,000 viable cells/vial • Capable of at least 16 population doublings • Mycoplasma: Not Detected • Hepatitis B: Not Detected • Hepatitis C: Not Detected • HIV-1: Not Detected • Bacteria, yeast, and other fungi: Not Detected
For Research Use Only. Not for use in diagnostic procedures.
規格
Donor SourceSingle Donor
No. of Cells≥500000
Product LineCascade Biologics™
Product TypeHuman Dermal Fibroblasts, Neonatal
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeNewborn (14 days or less)
Cell TypeFibroblast Cells (Dermal)
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Unit SizeEach
內容物與存放
Liquid nitrogen temperature
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常見問答集 (常見問題)
Can I get passage information for Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C)?
Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C) are primary human dermal fibroblasts isolated from adult skin and neonatal foreskin, respectively. They are cryopreserved at the end of the primary culture stage and not passaged.
What are your Human Dermal Fibroblast (HDF) cells grown in?
HDF cells are grown in Medium 106 with the addition of Low Serum Growth Supplement.
What media do you use during the first passage of primary cells?
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
How is the population doubling calculated for primary cells?
Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.
What are typical primary cell yields from tissue?
It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.