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The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.
The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production
The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.
The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production
The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.
For Research Use Only. Not for use in diagnostic procedures.
規格
Cell Line
FreeStyle™ 293-F Cells
Classification
Animal Origin-Free, Serum-Free
Delivery Type
Transfection
Culture Type
Suspension Cell Culture
Cell Type
293-derived Cells
Transfection Technique
Lipid-based Transfection, Transient Transfection
Expression Mechanism
Cell-Based Expression
Expression System
Mammalian
For Use With (Application)
Protein Expression
Key Functions
Protein Production
Product Line
Freestyle™
Product Type
Expression System
Quantity
1 Kit
內容物與存放
Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information.
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