Human Fibroblast Expansion Basal Medium (formerly “Medium 106”) is a sterile, liquid tissue culture medium intended for use as one component in a complete culture environment for the growth of normal human dermal fibroblasts. This basal culture medium contains:

• Essential and non-essential amino acids

• Vitamins

• Other organic compounds

• Trace minerals

• Inorganic salts. 

 

Human Fibroblast Expansion Basal Medium does NOT contain:

• Antibiotics

• Antimycotics

• Hormones

• Growth factors

• Proteins

 

This medium is HEPES and bicarbonate buffered and is designed for use in an incubator with an atmosphere of 5% CO²/95% air. To support the plating and long-term proliferation of human dermal fibroblasts, Human Fibroblast Expansion Basal Medium must be supplemented with Low Serum Growth Supplement (LSGS, Cat. No. S-003-10), or Low Serum Growth Supplement Kit (LSGS Kit, Cat. No. S-003-K).