The cell-permeant Magnesium Green™, AM exhibits a higher affinity for Mg²⁺ (K_d ∼1.0 mM) than mag-fura-2 (K_d ∼1.9 mM) or mag-indo-1 (K_d ∼2.7 mM). Upon binding Mg²⁺, Magnesium Green exhibits and increase in fluorescence emission intensity without a shift in wavelength with absorption/emission maxima ∼506/531 nm.

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